Premium
The development and validation of a high performance liquid chromatography (HPLC)/tandem mass spectrometry assay for fenticonazole in human plasma and comparison with an HPLC‐UV method
Author(s) -
Speed William,
Long Jeffrey M.,
Simmonds Roger J.,
Enos Trevor A.
Publication year - 1995
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290091424
Subject(s) - chemistry , chromatography , high performance liquid chromatography , tandem mass spectrometry , mass spectrometry , tandem , liquid chromatography–mass spectrometry , materials science , composite material
Abstract A method is described for the determination of fenticonazole in human female plasma. The method utilizes high performance liquid chromatography coupled to atmospheric pressure positive‐ion chemical ionization triple quadrupole mass spectrometry. Multiple reaction monitoring is employed for selectivity and sensitivity which enables quantification over the range 0.5–20 ng mL −1 with acceptable precision and accuracy. A comparison is made with an existing HPLC‐UV assay and the utility of the technology of combined liquid chromatography and tandem mass spectrometry for subnanogram per mL assays is discussed.