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An improved method for clenbuterol screening using high resolution selected ion recording
Author(s) -
Kingston R.,
Ordsmith N.,
Williams E.,
Thieme D.
Publication year - 1995
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290091414
Subject(s) - chemistry , clenbuterol , chromatography , resolution (logic) , derivatization , mass spectrometry , ion , analytical chemistry (journal) , high resolution , detection limit , remote sensing , artificial intelligence , organic chemistry , computer science , geology
A method has been developed using reliable GC derivatization techniques interfaced with a high resolution mass spectrometer. The method has proved successful for the detection of low levels (less than 1 ppm) of clenbuterol in complex biological matrices. Selected ion recording of two characteristic isotopic fragment ions provides a specific mode of detection by verifying the GC retention time of these ions and also by comparing their relative abundance. Analysis of urine samples demands higher mass spectrometric resolution, and 40000 (10% valley) was found to be a prerequisite for accurate integration of the drug‐related chromatographic peaks. The method developed is suitable for adaptation to a completely unattended automated routine incorporating sample injection, storage and retrieval of source tuning parameters, and data processing.