Premium
Collision‐induced dissociation of multiply charged peptides in an ion‐trap storage/reflectron time‐of‐flight mass spectrometer
Author(s) -
Qian Mark G.,
Zhang Yu,
Lubman David M.
Publication year - 1995
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290091311
Subject(s) - reflectron , chemistry , fragmentation (computing) , mass spectrometry , ion trap , time of flight , ion , dissociation (chemistry) , analytical chemistry (journal) , trap (plumbing) , electrospray ionization , ionization , time of flight mass spectrometry , atomic physics , chromatography , physics , organic chemistry , operating system , environmental engineering , engineering , computer science
Single‐frequency collisional activation of multiply charged peptides has been studied via electrospray ionization in an ion trap storage/reflectron time‐of‐flight device (IT/reTOF). Several peptides with molecular weights ranging from 600 to 1700 were used to demonstrate that sequence information can be obtained with this hybrid instrument with a sensitivity in the low picomole level. Further, a resolution of nearly 1000 can be obtained for the fragment products, due to the cooling effects of the buffer gas in the trap before analysis by the reTOF. The influence of the primary structure of the peptides on the observed collision‐induced fragmentation patterns is discussed. Although the current study is limited by the electronics, in terms of its ion isolation capabilities, it is demonstrated that the device has the potential for obtaining sequence information for peptides with excellent sensitivity and relatively high resolution.