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A simple method for differentiating Leu and Ile in peptides. The negative‐ion mass spectra of [M h] − ions of phenylthiohydantoin Leu and Ile
Author(s) -
Ramsay Steven L.,
Steinborner Simon T.,
Waugh Russell J.,
Dua Suresh,
Bowie John H.
Publication year - 1995
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290091305
Subject(s) - chemistry , mass spectrum , edman degradation , chromatography , ion , propane , derivative (finance) , peptide , amino acid , mass spectrometry , organic chemistry , peptide sequence , biochemistry , economics , financial economics , gene
Phenylthiohydantoin (PTH) amino acid derivatives are formed during sequential Edman degradation of peptides and proteins. Isomeric Leu and Ile (which differ only in respectively having iso ‐butyl and sec ‐butyl chains, and are often difficult to distinguish by conventional mass spectrometric techniques) may be readily identified by the characteristic decompositions of the [M H] − ions of their PTH derivatives. The Leu spectrum shows major loss of propane, while that of the Ile derivative shows elimination of both methane and ethane. This method may be used routinely with 10 μg quantities of peptide material.