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Endgroup analysis of polyethylene glycol polymers by matrix‐assisted laser desorption/ionization Fourier‐transform ion cyclotron resonance mass spectrometry
Author(s) -
de Koster Chris G.,
Duursma Marc C.,
van Rooij Gerard J.,
Heeren Ron M. A.,
Boon Jaap J.
Publication year - 1995
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290091018
Subject(s) - fourier transform ion cyclotron resonance , chemistry , mass spectrometry , analytical chemistry (journal) , matrix assisted laser desorption/ionization , ion cyclotron resonance , ion , desorption , chromatography , organic chemistry , cyclotron , adsorption
Abstract Fourier‐transform ion cyclotron resonance mass spectrometry (FTICR‐MS) by external injection of matrix‐assisted laser desorbed and ionized (MALDI) polymers offers good possibilities for characterization of low molecular weight homopolymers (MW range up to 10 kDa). The molecular masses of the molecular weight distribution (MWD) components of underivatized and derivatized (dimethyl, dipropyl, dibutyl and diacetyl) polyethylene giycol (PEG) 1000 and 4000 were measured by MALDI‐FTICR‐MS. These measurements have been performed using a commercial FTICR spectrometer with a home‐built external ion source. MALDI of the samples with a 2,5‐dihydroxybenzoic acid matrix in a 1000: 1 matrix‐to‐analyte molar ratio produces sodiated molecules in a sufficient yield to trap the ions in the ICR cell. The masses of the molecular weight distribution of PEG components were measured in broad‐band mode with a mass accuracy of < 5 ppm in the mass range around 1000 u and within 40 ppm accuracy around 4000 u. From these measurements, the endgroup mass of the polymer was determined by correlation of the measured component mass with the degree of polymerization. The masses of the PEG endgroups have been determined within a deviation of 3–10 millimass units for the PEG1000 derivatives and 10–100 millimass units for the PEG4000 derivatives, thus confirming the identity of the distal parts of the model compounds.