Quantitative analysis of cytokinins by electrospray tandem mass spectrometry | Zendy

Prinsen Els | Zendy; Redig Pascale | Zendy; Van Onckelen Henri A. | Zendy; Van Dongen Walter | Zendy; Esmans Eddy L. | Zendy

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Quantitative analysis of cytokinins by electrospray tandem mass spectrometry

Author(s) -

Prinsen Els,

Redig Pascale,

Van Onckelen Henri A.,

Van Dongen Walter,

Esmans Eddy L.

Publication year - 1995

Publication title -

rapid communications in mass spectrometry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.528

H-Index - 136

eISSN - 1097-0231

pISSN - 0951-4198

DOI - 10.1002/rcm.1290091016

Subject(s) - chemistry , chromatography , tandem mass spectrometry , electrospray , mass spectrometry , detection limit , quantitative analysis (chemistry) , selected reaction monitoring , resolution (logic) , tandem , electrospray mass spectrometry , analytical chemistry (journal) , materials science , artificial intelligence , computer science , composite material

A methodology was developed for the analysis of cytokinins from plant tissue using electrospray tandem mass spectrometry in combination with HPLC. A detection limit of 1 pmol zeatin‐riboside injected on‐column was achieved under multiple reaction monitoring conditions. Linearity was observed within a concentration range of 1–100 pmol injected on‐column. In view of the analysis of a considerable amount of samples, the chromatographic conditions were selected which were a compromise between speed of analysis and resolution. This approach, in combination with multiple reaction monitoring, allowed the analysis of up to 150 samples a day, and quantitation of 16 different cytokinins per sample.

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