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Quantitative analysis of cytokinins by electrospray tandem mass spectrometry
Author(s) -
Prinsen Els,
Redig Pascale,
Van Onckelen Henri A.,
Van Dongen Walter,
Esmans Eddy L.
Publication year - 1995
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290091016
Subject(s) - chemistry , chromatography , tandem mass spectrometry , electrospray , mass spectrometry , detection limit , quantitative analysis (chemistry) , selected reaction monitoring , resolution (logic) , tandem , electrospray mass spectrometry , analytical chemistry (journal) , materials science , artificial intelligence , computer science , composite material
A methodology was developed for the analysis of cytokinins from plant tissue using electrospray tandem mass spectrometry in combination with HPLC. A detection limit of 1 pmol zeatin‐riboside injected on‐column was achieved under multiple reaction monitoring conditions. Linearity was observed within a concentration range of 1–100 pmol injected on‐column. In view of the analysis of a considerable amount of samples, the chromatographic conditions were selected which were a compromise between speed of analysis and resolution. This approach, in combination with multiple reaction monitoring, allowed the analysis of up to 150 samples a day, and quantitation of 16 different cytokinins per sample.