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Distinguishing N‐ and C‐terminus ions for mass spectrometry sequencing of proteins without prior degradation
Author(s) -
Aaserud David J.,
Little Daniel P.,
O'Connor Peter B.,
McLafferty Fred W.
Publication year - 1995
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290091002
Subject(s) - chemistry , ion , dissociation (chemistry) , mass spectrometry , infrared multiphoton dissociation , electron capture dissociation , tandem mass spectrometry , fragmentation (computing) , ionization , mass spectrum , analytical chemistry (journal) , chromatography , organic chemistry , computer science , operating system
Identifying complementary pairs of fragment ions, b‐type vs y‐type, is a key step in mass spectral sequencing of proteins without degradation prior to ionization. Observations that b‐type ions tend to be less stable than y‐type have been extended to carbonic anhydrase (29kDa) as a model protein. Infrared multiphoton dissociation (IRMPD) of 24+, 27+, 30+ molecular ions shows y‐type ions from complementary pairs require, on average, 27% longer irradiation times for onset of abundance decrease, while in nozzle/skimmer dissociation of all charge states, y‐type ions require a 20% higher potential difference. In some cases, b‐ion dissociation appears to accompany its formation, as its rate of increase with increasing energy deposition is far less than that of its y‐complement.