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Negative‐ion electrospray mass spectra of peptides derivatized with 4‐aminonaphthalenesulphonic acid
Author(s) -
Griffiths William J.,
Lindh Ingemar,
Bergman Tomas,
Sjövall Jan
Publication year - 1995
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290090809
Subject(s) - chemistry , detector , electrospray , mass spectrometry , analytical chemistry (journal) , ion , mass spectrum , detection limit , spectral line , chromatography , optics , physics , organic chemistry , astronomy
The detection limits of a series of small peptides derivatized with 4‐aminonaphthalenesulphonic acid have been determined using a magnetic sector mass spectrometer operated in the negative‐ion mode. The instrument (AutoSpec‐QFPD) is equipped with an electrospray ion source and both array and point detectors. Detection limits when using the array detector were on the sub‐femtomole level at a mass resolution of 2000 (full width half maximum height). Cone‐voltage and linked‐scan collision‐induced dissociation spectra were recorded using both the array and point detectors. When using the array detector, amino acid sequence information could be obtained from as little as 50 fmol of sample consumed.