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Sensitive detection and structural characterization of trimethyl( p ‐aminophenyl)‐ammonium‐derivatized oligosaccharides by electrospray ionization–mass spectrometry and tandem mass spectrometry
Author(s) -
Okamoto Masahiko,
Takahashi KenIchi,
Doi Tadashi
Publication year - 1995
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290090804
Subject(s) - chemistry , electrospray ionization , mass spectrometry , fast atom bombardment , tandem mass spectrometry , protein mass spectrometry , chromatography , sample preparation in mass spectrometry , oligosaccharide , reductive amination , selected reaction monitoring , electrospray , extractive electrospray ionization , analytical chemistry (journal) , organic chemistry , catalysis
Mass spectrometric methodologies which use electrospray ionization (ESI) for oligosaccharide structural determination and which are linked to classical procedures, such as reductive amination, have been established. Several derivatives of a model oligosaccharide were prepared and their ionization efficiencies in electrospray ionization (ESI) and fast‐atom bombardment (FAB) mass spectrometry were investigated. The trimethyl‐( p ‐aminophenyl)ammonium (TMAPA) derivatives showed extremely high sensitivity in positive‐ion ESI–MS and gave Y‐ and Z‐series sequence ions by ESI tandem mass spectromety (MS/MS). This study suggests that TMAPA‐derivatized oligosaccharides used in ESI‐MS and ESI‐MS/MS would be effective for structural characterization of oligosaccharides that are available only in limited quantities.