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A study of factors influencing the formation of singly charged oligomers and multiply charged monomers by matrix‐assisted laser desorption/ionization mass spectrometry
Author(s) -
Zhong Feng,
Zhao Shankai
Publication year - 1995
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290090705
Subject(s) - chemistry , monomer , mass spectrometry , matrix (chemical analysis) , substrate (aquarium) , ionization , analytical chemistry (journal) , mass spectrum , desorption , matrix assisted laser desorption/ionization , ion , photochemistry , chromatography , polymer , organic chemistry , oceanography , adsorption , geology
The formation of singly charged oligomers and multiply charged monomers has been studied under different conditions with matrix‐assisted laser desorption/ionization and it has been found that the concentration of analyte in matrix solution, as well as the matrix and substrate used, are factors that influence this process. The concentration of analyte in the matrix solution has a strong influence on the formation of singly charged oligomers. When α‐cyano‐4‐hydroxycinnamic acid is used as matrix, more doubly charged monomeric molecular ions are observed than when sinapinic acid is used. By using a nitrocellulose membrane instead of a silver plate as substrate, we find more multiply charged monomers and dimers in the spectra, especially in the case of high mass proteins, such as albumin. It seems that the formation of multiply charged monomers and oligomers is influenced by the overall amount of charge that the sample carries.