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Structural characterization of glutaminergic blocker spider toxins by high‐energy collision charge‐remote fragmentations
Author(s) -
Fujita Tsuyoshi,
Itagaki Yasuhiro,
Naoki Hideo,
Nakajima Terumi,
Hagiwara Ken'ichi
Publication year - 1995
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290090502
Subject(s) - chemistry , ion , dissociation (chemistry) , spider toxin , tandem mass spectrometry , collision induced dissociation , protonation , fast atom bombardment , tandem , mass spectrum , mass spectrometry , spider , analytical chemistry (journal) , organic chemistry , chromatography , glutamate receptor , biochemistry , zoology , receptor , materials science , composite material , biology
Abstract Spider venom gland stores various glutaminergic blockers such as acylpolyamines, besides proteins and peptides. A method is described for structure determination of these acylpolyamines using fast‐atom bombardment and four‐sector tandem mass spectrometry. The collision‐induced dissociation (CID) spectra of protonated molecular ions of synthetic model compounds show only a small number of weak, sequence‐related product ions. But, unlike the CID spectra of [M + H] + ions, dissociation of sodium‐attached molecular ions leads to charge‐remote fragmentations and provides intense sequence‐related product ions.