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Liquid chromatography/mass spectrometry with collision‐induced dissociation of arachidonic acid metabolites derivatized with aminobenzenesulphonic acid
Author(s) -
Yang Y.,
Griffiths W. J.,
Lindgren J. Å.,
Sjövall J.
Publication year - 1995
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290090407
Subject(s) - chemistry , chromatography , dissociation (chemistry) , fast atom bombardment , mass spectrometry , arachidonic acid , ion , collision induced dissociation , mass spectrum , fatty acid , structural isomer , tandem mass spectrometry , organic chemistry , enzyme
A fused‐silica capillary high‐performance liquid chromatography column (i.d. 0.25 mm) packed with octadecylsilane‐bonded silica has been used on‐line with continuous‐flow negative‐ion fast‐atom bombardment (FAB) mass spectrometry for the separation and structural analysis of series of fatty acids and arachidonic acid metabolites derivatized with 4‐aminobenzenesulphonic acid. Negative‐ion FAB spectra of the derivatized lipids showed strong [MH] − pseudomolecular ions. Collision‐induced dissociation of these anions produced structurally informative daughter ions, arising from charge‐remote fragmentations.