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Immobilized human serum albumin: Liquid chromatography/mass spectrometry as a method of determining drug–protein binding
Author(s) -
Tiller Philip R.,
Mutton Ian M.,
Lane Stephen J.,
Bevan Christopher D.
Publication year - 1995
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290090403
Subject(s) - chemistry , chromatography , mass spectrometry , liquid chromatography–mass spectrometry , human serum albumin , albumin , drug , serum albumin , plasma protein binding , biochemistry , pharmacology , medicine
A liquid chromatography/mass spectrometry methodology is described that enables the simultaneous estimation of the individual protein‐binding affinities of a mixture of compounds. This approach has proved to be robust and enables a series of molecules to be ranked according to their protein‐binding affinities within 20 minutes.