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Studies on non‐covalent associations of immunosuppressive drugs with serum albumin using pneumatically assisted electrospray ionization mass spectrometry
Author(s) -
Bakhtiar Ray,
Stearns Ralph A.
Publication year - 1995
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290090313
Subject(s) - chemistry , electrospray ionization , chromatography , mass spectrometry , electrospray , serum albumin , albumin , biochemistry
Pneumatically assisted electrospray ionization (ion‐spray) mass spectrometry was employed in the detection of non‐covalent interactions of immunosuppressive drugs with proteins. Non‐covalent complexes of albumin (rat and bovine) with FK506, dihydro‐FK506, and FK520 were observed. No complexation was detected between albumin and cyclosporin A (CsA), even at a protein‐ligand molar ratio of 1:20. It is suggested that the lack of detection of an albumin–CsA non‐covalent complex may be attributed to dissociation of the weakly associated species during ion vaporization. Nonetheless, preliminary results indicate that ion‐spray mass spectrometry has potential in qualitative evaluation of drug–plasma protein interaction.