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Matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry of DNA–Pt(II) complexes
Author(s) -
Guittard J.,
Pacifico C.,
Blais J. C.,
Bolbach G.,
Chottard J. C.,
Spassky A.
Publication year - 1995
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290090109
Subject(s) - chemistry , mass spectrometry , ionization , desorption , matrix (chemical analysis) , matrix assisted laser desorption electrospray ionization , analytical chemistry (journal) , chromatography , thermal ionization mass spectrometry , ion , organic chemistry , adsorption
Matrix‐assisted laser desorption ionization (MALDI) time‐of‐flight mass spectrometry has been used to characterize the reaction products of the 18‐mer deoxyribonucleotide d(AACGGTTAACCGTTAATT) with [Pt(NH 3 ) 3 (H 2 O)] 2+ and cis ‐[Pt(NH 3 ) 2 (H 2 O) 2 ] 2+ . Characteristic peaks corresponding to different monofunctional adducts {18‐mer+ n [Pt(NH 3 ) 3 ]} ( n =1, 2, 3 and 4) have been observed with the triamino–monoaqua complex. With the diamino‐diaqua cis ‐Pt complex, formation of a chelate {18‐mer+[Pt(NH 3 ) 2 ]} involving two adjacent guanines has been demonstrated. A good correlation between MALDI and polyacrylamide gel electrophoresis results is observed.