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Electrospray mass spectrometry for the characterization of the purity of natural and modified oligodeoxynucleotides
Author(s) -
Deroussent Alain,
Le Caer JeanPierre,
Rossier Jean,
Gouyette Alain
Publication year - 1995
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290090102
Subject(s) - chemistry , electrospray ionization , mass spectrometry , chromatography , electrospray , electrospray mass spectrometry , sample preparation in mass spectrometry , extractive electrospray ionization , adduct , protein mass spectrometry , analytical chemistry (journal) , organic chemistry
Electrospray ionization mass spectrometry is an accurate and sensitive analytical method to characterize the purity of oligodeoxynucleotides being tested for pharmacological studies. We report the preparation procedure (‘desalting’) of natural and modified oligodeoxynucleotides (ODNs) and their analysis by negative‐ion electrospray mass spectrometry. We evaluate the sensitivity and the accuracy of the method for two antisense ODN sequences. Mass analysis of the 25‐mer phosphorothioate can be performed to within 0.001% accuracy (standard error of 0.05 Da) for a sample concentration of 12 pmol/μL. In addition, the adduct ion and the failure sequence can be identified to characterize the antisense ODN.