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Differentiation of bacteria using protein profiles from matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry
Author(s) -
Cain Teresa C.,
Lubman David M.,
Weber Walter J.
Publication year - 1994
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290081224
Subject(s) - chemistry , mass spectrometry , matrix assisted laser desorption/ionization , sonication , chromatography , desorption , matrix assisted laser desorption electrospray ionization , matrix (chemical analysis) , sample preparation , substrate (aquarium) , surface enhanced laser desorption/ionization , ionization , analytical chemistry (journal) , sample preparation in mass spectrometry , time of flight mass spectrometry , electrospray ionization , organic chemistry , ion , oceanography , adsorption , geology
Matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry is demonstrated as a means for rapidly differentiating bacteria based upon their water‐soluble‐protein profiles. The procedure involves release of cell contents by breaking cell membranes using a sonication method, and is performed with minimal sample preparation. Minimal purification of cell contents is required for the procedure. Protein profile signals obtained are found to be enhanced greatly by the use of a Nafion substrate.