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2‐(4‐hydroxyphenylazo)‐benzoic acid: A solid matrix for matrix‐assisted laser desorption/ionization of polystyrene
Author(s) -
Montaudo Giorgio,
Montaudo Maurizio S.,
Puglisi Concetto,
Samperi Filippo,
Daolio S.
Publication year - 1994
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290081221
Subject(s) - chemistry , polystyrene , benzoic acid , desorption , matrix (chemical analysis) , analytical chemistry (journal) , matrix assisted laser desorption/ionization , ionization , mass spectrometry , laser , mass spectrum , spectral line , polymer , chromatography , organic chemistry , optics , ion , physics , adsorption , astronomy
Liquid matrices are currently used in matrix assisted laser desorption ionization (MALDI) of non‐polar macromolecules, such as polystyrene. We investigated the possibility of using non‐liquid compounds as matrices. A series of polystyrene samples was analyzed by MALDI using a time‐of‐flight (TOF) analyzer. We found that a solid matrix, namely 2‐(4‐hydroxyphenylazo)‐benzoic acid, yields quality mass spectra. The MALDI‐TOF spectra were used to measure the parameters which characterize the molecular weight distributions of the polystyrene samples. The values obtained by MALDI‐TOF were in good agreement with those obtained by other methods.