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3‐aminopicolinic aid as a matrix for laser desorption mass spectrometry of biopolymers
Author(s) -
Taranenko N. I.,
Tang K.,
Allman S. L.,
Ch'ang L. Y.,
Chen C. H.
Publication year - 1994
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290081219
Subject(s) - chemistry , mass spectrometry , picolinic acid , dna , desorption , matrix (chemical analysis) , matrix assisted laser desorption/ionization , ion , matrix assisted laser desorption electrospray ionization , analytical chemistry (journal) , chromatography , biochemistry , organic chemistry , adsorption
3‐Aminopicolinic acid (3‐APA) was tested and found to be a useful matrix for matrix‐assisted laser desorption/ionization of DNA and protein. Single‐stranded DNA segments of 150‐mer and double‐stranded DNA of 246 base pairs were successfully detected by using 3‐APA as an ultraviolet‐absorbing matrix in a linear time‐of‐flight mass spectrometer. In the case of the double‐stranded DNA, only parent ions corresponding to single‐stranded DNA were observed. The comparison with 3‐hydroxypicolinic acid and picolinic acid matrices is discussed.