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Conjugation reactions of cyclodepsipeptide to glutathionyl adducts by direct ‘in‐beam’ synthesis under negative‐ion fast‐atom bombardment conditions
Author(s) -
Loutelier C.,
Cherton J.C.,
Lange C.
Publication year - 1994
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290081010
Subject(s) - chemistry , adduct , fast atom bombardment , glutathione , mass spectrometry , substrate (aquarium) , ion , stereochemistry , organic chemistry , chromatography , enzyme , oceanography , geology
The conjugation products of E‐destruxin (cyclodepsipeptide) with glutathione have been identified by fast‐atom bombardment mass spectrometry. Appropriate conditions were developed for cyclodepsipeptide substrate, which enabled the direct, dynamic mass spectra analysis of spontaneous as well as glutathione‐s‐transferase catalyzed conjugation reactions. Application to the most active cyclopeptide in the series of destruxins yielded glutathionyl adduct.