Optimization by mass spectrometry of a tryptophan‐specific protein cleavage reaction | Zendy

Vestling Martha M. | Zendy; Kelly Michele A. | Zendy; Fenselau Catherine | Zendy; Costello Catherine E. | Zendy

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Optimization by mass spectrometry of a tryptophan‐specific protein cleavage reaction

Author(s) -

Vestling Martha M.,

Kelly Michele A.,

Fenselau Catherine,

Costello Catherine E.

Publication year - 1994

Publication title -

rapid communications in mass spectrometry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.528

H-Index - 136

eISSN - 1097-0231

pISSN - 0951-4198

DOI - 10.1002/rcm.1290080925

Subject(s) - chemistry , cleavage (geology) , tryptophan , mass spectrometry , side chain , chromatography , organic chemistry , biochemistry , amino acid , polymer , geotechnical engineering , fracture (geology) , engineering

Mass spectrometry has been used to define the reaction of BNPS‐skatole (3‐bromo‐3‐methyl‐2‐( o ‐nitrophenylsulfenyl)indolenine) with selected proteins and peptides, confirming the cleavage of the protein chain at tryptophan residues, characterizing the products, and identifying the side reactions. A short procedure for carrying out the cleavage reaction with minimal side reactions is presented.

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