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Optimization by mass spectrometry of a tryptophan‐specific protein cleavage reaction
Author(s) -
Vestling Martha M.,
Kelly Michele A.,
Fenselau Catherine,
Costello Catherine E.
Publication year - 1994
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290080925
Subject(s) - chemistry , cleavage (geology) , tryptophan , mass spectrometry , side chain , chromatography , organic chemistry , biochemistry , amino acid , polymer , geotechnical engineering , fracture (geology) , engineering
Mass spectrometry has been used to define the reaction of BNPS‐skatole (3‐bromo‐3‐methyl‐2‐( o ‐nitrophenylsulfenyl)indolenine) with selected proteins and peptides, confirming the cleavage of the protein chain at tryptophan residues, characterizing the products, and identifying the side reactions. A short procedure for carrying out the cleavage reaction with minimal side reactions is presented.