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Detection of 500‐nucleotide DNA by laser desorption mass spectrometry
Author(s) -
Tang K.,
Taranenko N. I.,
Allman S. L.,
Cháng L. Y.,
Chen C. H.,
Lubman D. M.
Publication year - 1994
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290080913
Subject(s) - chemistry , mass spectrometry , chromatography , desorption , dna , nucleotide , analytical chemistry (journal) , biochemistry , organic chemistry , gene , adsorption
We report the first detection of DNA segments as large as 500 nucleotides by matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry, using picolinic acid and 3‐hydroxypicolinic acid mixtures as desorption matrices. The successful detection of 500‐nucleotide DNA indicates that laser mass spectrometry is now emerging as a new biotechnology tool for DNA‐related research. It should be possible to utilize fast detection of large DNA segments by laser mass spectrometry for rapid human genome sequencing.