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Matrix‐assisted laser desorption/ionization mass sectrometry of restriction enzyme‐digested plasmid DNA using an active nafion substrate
Author(s) -
Bai Jian,
Liu YanHui,
Lubman David M.,
Siemieniak David,
Chen ChungHsuan
Publication year - 1994
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290080904
Subject(s) - chemistry , substrate (aquarium) , desorption , nafion , matrix assisted laser desorption electrospray ionization , dna , ionization , plasmid , soft laser desorption , mass spectrometry , chromatography , restriction enzyme , matrix (chemical analysis) , matrix assisted laser desorption/ionization , analytical chemistry (journal) , biochemistry , organic chemistry , ion , oceanography , electrode , adsorption , electrochemistry , geology
Matrix‐assisted laser desorption/ionization using a 3‐hydroxpicollinic acid matrix from an active Nafion substrate has been used for detection of restriction enzyme‐degested double‐stranded plasmid DNA using time‐of‐ flight mass spectrometry. DNA strands of up to 267 base pairs were detected with minimal sample purification, although only as species corresponding to single‐stranded DNA.