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Matrix‐assisted laser desorption/ionization capabilities in the study of non‐enzymatic protein glycation
Author(s) -
Lapolla Annunziata,
Fedele Domenico,
Seraglia Roberta,
Catinella Silvia,
Traldi Pietro
Publication year - 1994
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290080815
Subject(s) - chemistry , glycation , matrix assisted laser desorption/ionization , desorption , surface enhanced laser desorption/ionization , maillard reaction , mass spectrometry , enzyme , bovine serum albumin , albumin , chromatography , biochemistry , glycosylation , fructose , matrix (chemical analysis) , adsorption , organic chemistry , receptor
Matrix‐assisted laser desorption/ionization mass spectrometry has been succesfully applied in the study of non‐enzymatic glycation of different proteins. In the case of bovine serum albumin, glycated by in vitro experiments performed under pseudophysiological conditions, a clear increase in molecular weight is observed with respect to both glucose concentrations and incubation time. The in vitro glycation of ribonuclease with glucose and fructose shows some peculiar differences either in terms of the number of condensed sugar molecules or in terms of the reaction kinetics. The same approach, applied to plasma proteins of healthy and diabetic subjects, provides evidence for the occurence of glycation of human serum albumin for the latter subjects.