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Development of packed capillary column electrochromatography/mass spectrometry
Author(s) -
Gordon Derek B.,
Lord Gwyn A.,
Jones David S.,
Games David E.
Publication year - 1994
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290080711
Subject(s) - chemistry , chromatography , capillary electrochromatography , mass spectrometry , high performance liquid chromatography , electrokinetic phenomena , electrochromatography , capillary action , analyte , micellar electrokinetic chromatography , elution , analytical chemistry (journal) , supercritical fluid chromatography , stationary phase , capillary electrophoresis , materials science , composite material
The enhanced performance of electro‐osmotically driven high performance liquid chromatography (HPLC) using reversed‐phase packed capillaries has been evaluated for coupling to mass spectrometers, as an alternative to both conventional pressure‐driven elution, and to micellar electrokinetic capillary chromatography for the separation of neutral analytes. An off‐line comparison of electro‐osmotically driven versus pressure driven chromatography for the separation of an aromatic mixture shows the superiority of the method over conventional HPLC. Coupling of the technique with continuous‐flow fast‐atom bombardment for the analysis of a steroid mixture is described.