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Tryptic peptide mapping of sequence 1–298 of human serum albumin by high‐performance liquid chromatography and fast‐atom bombardment mass spectrometry
Author(s) -
Compagnini Anna,
Fisichella Salvatore,
Foti Salvatore,
Saletti Rosaria,
Sardo Letizia
Publication year - 1994
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290080606
Subject(s) - chemistry , fast atom bombardment , chromatography , mass spectrometry , peptide , albumin , sequence (biology) , high performance liquid chromatography , protein mass spectrometry , tandem mass spectrometry , biochemistry
The determination of the tryptic peptide mapping of sequence 1–298 of human serum albumin (HSA) by chemical and enzymatic cleavage and combined use of high‐performance liquid chromatography (HPLC) and fast‐atom bombardment mass spectrometry (FAB‐MS) is described. A total coverage of about 75% of the entire sequence was obtained. Unidentified fragments included some peptides which were not present in the chromatograms because of their extreme hydrophobic or hydrophilic character, as indicated by the calculated retention times. Due to the high reproducibility of the experiments and to the satisfactory yield of the tryptic fragments identified, the combined use of HPLC and FAB‐MS appears to possess great potential for structural investigation of HSA.