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Characterization of hematology lysing reagents containing quaternary ammonium halides using fast‐atom bombardment mass spectrometry
Author(s) -
BambagiottiAlberti Massimo,
Coran Silvia,
Giannellini Valerio,
Favretto Donata,
Traldi Pietro
Publication year - 1994
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290080602
Subject(s) - chemistry , fast atom bombardment , reagent , halide , mass spectrometry , ammonium , radiochemistry , chromatography , inorganic chemistry , organic chemistry
Positive‐ and negative‐ion fast‐atom bombardment (FAB) mass spectrometry has been employed to characterize pure dodecyl‐, tetradecyl‐ and hexadecyl‐trimethylammonium chlorides and bromides, compounds of high interest as lysing agents in hematologic analyses. In parallel, the same approach has been used for some commercially available lysing reagent products. While in positive‐ion FAB mass spectra the presence of the abundant ions corresponding to the intact quaternary ammonium cations (Q + ) is accompanied by the presence of [Q 2 X] + (X = Cl, Br) species, in the negative‐ion mode cluster ions such as [QX 2 ] − are the sole ionic species that are easily evident. The mechanism for the formation of [Q 2 X] + and [QX 2 ] − species has been studied and some suggested explanations are given.