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Formation of multiply charged ions from large molecules using massive‐cluster impact
Author(s) -
Mahoney John F.,
Shan Cornett D.,
Lee Terry D.,
Barofsky Douglas F.
Publication year - 1994
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290080513
Subject(s) - chemistry , mass spectrometry , ion , cluster (spacecraft) , ionization , fast atom bombardment , quadrupole mass analyzer , mass spectrum , electron ionization , quadrupole , molecule , thermal ionization mass spectrometry , analytical chemistry (journal) , range (aeronautics) , secondary ion mass spectrometry , atomic physics , chromatography , physics , organic chemistry , materials science , computer science , composite material , programming language
Massive‐cluster impact is demonstrated to be an effective ionization technique for the mass analysis of proteins as large as 17 kDa. The design of the cluster source permits coupling to both magnetic‐sector and quadrupole mass spectrometers. Mass spectra are characterized by the almost total absence of chemical background and a predominance of multiply charged ions formed from 100% glycerol matrix. The number of charge states produced by the technique is observed to range from +3 to +9 for chicken egg lysozyme (14 310 Da). The lower m/z values provided by higher charge states increase the effective mass range of analyses performed with conventional ionization by fast‐atom bombardment or liquid secondary ion mass spectrometry.