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Simple and sensitive quantitation method for mevalonic acid in plasma using gas chromatography/mass spectrometry
Author(s) -
Ishihama Yasushi,
Mano Nariyasu,
Oda Yoshiya,
Hiyoshi Hironobu,
Ikuta Hironori,
Yoshida Yutaka
Publication year - 1994
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290080507
Subject(s) - chemistry , chromatography , mass spectrometry , isobutane , extraction (chemistry) , chloroform , gas chromatography , gas chromatography–mass spectrometry , organic chemistry , catalysis
The combination of gas chromatography and mass spectrometry using a polar capillary column and isobutane chemical ionization made it possible to determine mevalonic acid (MVA) as the lactone at subnanogram levels using 1 mL of plasma. The pretreatment procedure consisted of only three steps, namely lactonization, washing with chloroform and liquid/liquid extraction. This simple, rapid and sensitive method, having good precision and accuracy, is useful for evaluating the change of plasma MVA, which is well correlated with whole‐body cholesterol biosynthesis.