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The application of electrospray and neutral‐loss mass spectrometry to the identification of the metabolites of S12813 in rat liver slices
Author(s) -
Brownsill Robin,
Combal JeanPhilippe,
Taylor Albert,
Bertrand Marc,
Luijten Wim,
Walther Bernard,
Games D. E.
Publication year - 1994
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290080504
Subject(s) - chemistry , chromatography , electrospray ionization , mass spectrometry , glucuronide , electrospray mass spectrometry , tandem mass spectrometry , electrospray , metabolism , protein mass spectrometry , biochemistry
The metabolism of S12813, (3‐{2‐[4‐phenyl piperazin‐1‐yl] ethyl}‐2‐oxo‐2,3‐dihydro oxazolo [4, 5‐b] pyridine chlorohydrate), in rat liver slice incubates was examined by high performance liquid chromatography combined with mass spectrometry. Electrospray ionization was used together with tandem mass spectrometric techniques of analysis (MS/MS). Polar phase I and phase II metabolites were identified as C‐oxidation products, which were then conjugated to form either sulphate or glucuronide metabolites. On the basis of the identifications made, a metabolic pathway of S12813 in rat liver slices has been proposed.