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Analysis of neuropeptides by perfusion liquid chromatography/electrospray ion‐trap mass spectrometry
Author(s) -
Lin HungYu,
Voyksner Robert D.
Publication year - 1994
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290080411
Subject(s) - chemistry , chromatography , electrospray , mass spectrometry , electrospray ionization , high performance liquid chromatography , ion trap , neuropeptide , biochemistry , receptor
Abstract Perfusion high‐performance liquid chromatography (HPLC) combined with electrospray ion trap mass spectrometry (ITMS) was evaluated for the determination of neuropepetides in plasma. Perfusion HPLC offers the capability of resolving neuropeptides spiked into plasma in 5 min compared to the 30–60 min separations performed on packed capillary C 18 columns. Electrospray combined with the ITMS provides the ability to ionize these neuropeptides and mass analyze them with high sensitivity and specificity. Sub‐picomole quantities of neuropeptides injected on‐column could be specifically detected in a plasma matrix. The electrospray‐ITMS mass spectrum of each neuropeptide showed multiply charged ions which could be used to determine or confirm their molecular weights.