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Gas chromatography/combustion/isotope‐ratio mass spectrometry analysis of urinary steroids to detect misuse of testosterone in sport
Author(s) -
Becchi Michel,
Aguilera Rodrigue,
Farizon Yves,
Flament MarieMagdeleine,
Casabianca Hervé,
James Patrick
Publication year - 1994
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290080404
Subject(s) - chemistry , epitestosterone , testosterone (patch) , urine , chromatography , mass spectrometry , isotope ratio mass spectrometry , gas chromatography , isotope , gas chromatography–mass spectrometry , androgen , endocrinology , biochemistry , hormone , medicine , physics , quantum mechanics
We propose a new confirmatory method for testosterone doping in sport. The present method in use, based on measuring the testosterone/epitestosterone (T/E) ratio in urine, may miss suspicious cases, or lead to reporting cases in which the high ratio is natural. Synthetic testosterone has a 13 C abundance different from that of endogenous human testosterone. The connection of a gas chromatograph to an isotope‐ratio mass spectrometer via a combustion interface allows the measurement of the corresponding characteristic value (δ‰) for testosterone, its precursors, and its metabolites. To detect exogenous administration of testosterone, 30–40 mL of urine is sufficient.