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Charge‐remote fragmentation of sulphated and glucuronidated bile acids and their 2‐aminoethanesulphonic acid derivatives
Author(s) -
Griffiths William J.,
Zhang Jie,
Sjövall Jan
Publication year - 1994
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290080302
Subject(s) - chemistry , fast atom bombardment , fragmentation (computing) , dissociation (chemistry) , collision induced dissociation , taurine , ion , mass spectrum , bile acid , mass spectrometry , amino acid , conjugated system , tandem mass spectrometry , chromatography , organic chemistry , biochemistry , polymer , computer science , operating system
Negative‐ion fast‐atom bombardment mass spectra of bile acids show prominent [M—H] − pseudomolecular ions, and few fragment ions. Collision‐induced dissociation of such pseudomolecular ions gives charge‐remote fragmentations which are useful in structural identifications. It has been shown that bile acids conjugated with taurine (2‐aminoethanesulphonic acid) give more intense and informative collision‐induced dissociation spectra than unconjugated bile acids. In this laboratory, a procedure for the aminosulphation of biological samples, for analysis by tandem mass spectrometry, has been developed. In the present study, a comparison has been made between the collision‐induced dissociation spectra of a series of sulphated and glucuronidated bile acids and their 2‐aminoethanesulphonic acid derivatives.