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Characterization of glucuronic acid conjugates of a novel angiotensin receptor antagonist
Author(s) -
Bowers Gary D.,
Eddershaw Peter J.,
Hughes Soraya Y.,
Manchee Gary R.,
Oxford Janet,
Games D. E.
Publication year - 1994
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290080218
Subject(s) - chemistry , chromatography , mass spectrometry , glucuronic acid , conjugate , high performance liquid chromatography , thermospray , tandem mass spectrometry , biochemistry , selected reaction monitoring , polysaccharide , mathematical analysis , mathematics
Nanogram quantities of glucuronic acid conjugates of GR117289 in rat and dog bile have been analysed by semi‐microbore high‐performance liquid chromatography (HPLC)/ionspray mass spectrometry with on‐line UV diode array detection. The determination of drug metabolites in bile has often proved problematical due to the large number of endogenous components in this biological matrix, in particular the bile acids. Semi‐microbore HPLC is useful for concentrating small quantities of material and, in combination with an on‐line diode array detector, for distinguishing between drug related and endogenous components. A novel angiotensin II receptor antagonist, GR117289, had proved difficult to analyse by thermospray mass spectrometry because of its thermal lability. The use of the less thermally dependent technique of ionspray mass spectrometry allowed the characterization of nanogram quantities of glucuronic acid metabolites of GR117289 in bile.