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Observation of large multimers in the electrospray ionization mass spectrometry of peptides
Author(s) -
Busman Mark,
Knapp Daniel R.,
Schey Kevin L.,
Smith Richard
Publication year - 1994
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290080217
Subject(s) - chemistry , electrospray ionization , mass spectrometry , electrospray , analytical chemistry (journal) , quadrupole , chromatography , quadrupole mass analyzer , protein mass spectrometry , biomolecule , substrate (aquarium) , ionization , ion , organic chemistry , biochemistry , physics , oceanography , atomic physics , geology
Large multimeric ions were detected from electrospray ionization (ESI) mass spectrometry of peptides, using a quadrupole mass spectrometer of standard mass range. Multimers, up to heptamers, were seen for angiotensin I; and multimers, up to pentamers, were observed for renin substrate. The appearance of the multimeric species could be controlled by manipulation of sampling conditions in the ESI interface. The relevance of the observation of large multimers to proposed mechanisms of ESI is discussed. Implications of the observation of large multimers to efforts to deduce higher order structure of biomolecules from ESI mass spectrometric experiments are also noted.