Quantitative analysis of low molecular weight compounds of biological interest by matrix‐assisted laser desorption ionization | Zendy

Duncan Mark W. | Zendy; Matanovic Gabrijela | Zendy; CerpaPoljak Anne | Zendy

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Quantitative analysis of low molecular weight compounds of biological interest by matrix‐assisted laser desorption ionization

Author(s) -

Duncan Mark W.,

Matanovic Gabrijela,

CerpaPoljak Anne

Publication year - 1993

Publication title -

rapid communications in mass spectrometry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.528

H-Index - 136

eISSN - 1097-0231

pISSN - 0951-4198

DOI - 10.1002/rcm.1290071207

Subject(s) - chemistry , internal standard , mass spectrometry , desorption , analyte , quantitative analysis (chemistry) , chromatography , matrix assisted laser desorption/ionization , matrix (chemical analysis) , analytical chemistry (journal) , peptide , ionization , acetylcholine , ion , organic chemistry , biochemistry , medicine , adsorption , endocrinology

Internal standards were used to demonstrate that matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry can be applied to the quantitative analysis of low molecular weight polar compounds. Three examples were tested: a standard curve for 3,4‐dihydroxyphenylalanine (DOPA) was prepared using a stable isotope analogue (i.e., [ 13 C 6 ]DOPA) as an internal standard; [ 2 H 16 ]‐acetylcholine was employed as an internal standard for the quantification of acetylcholine; and in the final example, the peptide Ac‐Ser‐Ile‐Arg‐His‐Tyr‐NH 2 was used as an internal standard for the quantification of the peptide H‐Ser‐Ala‐Leu‐Arg‐His‐Tyr‐NH 2 . In each instance, straight line fits ( r 2 >0.95) demonstrate that MALDI is a viable approach for the quantitative analysis of low molecular weight analytes.

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