Characterization of succinimide‐type dehydration products of recombinant hirudin variant 1 by electrospray tandem mass spectrometry | Zendy

Grossenbacher H. | Zendy; Märki W. | Zendy; Coulot M. | Zendy; Müller D. | Zendy; Richter W. J. | Zendy

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Characterization of succinimide‐type dehydration products of recombinant hirudin variant 1 by electrospray tandem mass spectrometry

Author(s) -

Grossenbacher H.,

Märki W.,

Coulot M.,

Müller D.,

Richter W. J.

Publication year - 1993

Publication title -

rapid communications in mass spectrometry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.528

H-Index - 136

eISSN - 1097-0231

pISSN - 0951-4198

DOI - 10.1002/rcm.1290071205

Subject(s) - chemistry , succinimide , dehydration , tandem mass spectrometry , electrospray , tandem , electrospray mass spectrometry , mass spectrometry , chromatography , characterization (materials science) , electrospray ionization , organic chemistry , biochemistry , nanotechnology , materials science , composite material

Two congeners Q 4 and Q 5 inferred in earlier analyses to be cyclic succinimide‐type dehydration product; of recombinant hirudin (variant 1) were structurally fully characterized. After isotopic labeling by ring‐opening with H 2 18 O, the suspected anhydro‐positions Asp 53 and Asp 33 were confirmed by tandem mass spectrometry (MS/MS) sequencing of relevant smaller peplides directly in the enzymatic hydrolysates (V8 protease) using electrospray MS/MS. The chosen strategy proved highly efficient and sensitive.

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