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Characterization of succinimide‐type dehydration products of recombinant hirudin variant 1 by electrospray tandem mass spectrometry
Author(s) -
Grossenbacher H.,
Märki W.,
Coulot M.,
Müller D.,
Richter W. J.
Publication year - 1993
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290071205
Subject(s) - chemistry , succinimide , dehydration , tandem mass spectrometry , electrospray , tandem , electrospray mass spectrometry , mass spectrometry , chromatography , characterization (materials science) , electrospray ionization , organic chemistry , biochemistry , nanotechnology , materials science , composite material
Two congeners Q 4 and Q 5 inferred in earlier analyses to be cyclic succinimide‐type dehydration product; of recombinant hirudin (variant 1) were structurally fully characterized. After isotopic labeling by ring‐opening with H 2 18 O, the suspected anhydro‐positions Asp 53 and Asp 33 were confirmed by tandem mass spectrometry (MS/MS) sequencing of relevant smaller peplides directly in the enzymatic hydrolysates (V8 protease) using electrospray MS/MS. The chosen strategy proved highly efficient and sensitive.