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Quantification of abscisic acid using liquid chromatography/thermospray ionization tandem mass spectrometry for selected reaction monitoring
Author(s) -
Okamoto Masahiko,
Takahashi Kenichi,
Doi Tadashi
Publication year - 1993
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290071202
Subject(s) - thermospray , chemistry , chromatography , derivatization , abscisic acid , mass spectrometry , liquid chromatography–mass spectrometry , tandem mass spectrometry , detection limit , selected reaction monitoring , chemical ionization , high performance liquid chromatography , ionization , ion , organic chemistry , biochemistry , gene
A liquid chromatography/thermospray ionization tandem mass spectrometry (LC/TSP‐MS/MS) method was developed for the quantification of abscisic acid (ABA) in crude plant extracts without derivatization. By this technique interfering chemical background ions are eliminated and a detection limit for ABA olf 0.2 ng is achieved. Reproducibility for quantification is attained by parallel reaction monitoring of ABA and added ABA‐d 6 used as an internal standard. The standard curve is linear over the range 200 pg to 10 ng of injected ABA. As examples of the application of the methodology, the quantification of ABA in some plant tissues is given.