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Analysis of the squalestatins by on‐line packed capillary liquid chromatography combined with electrospray mass spectrometry
Author(s) -
Lane S. J.,
Brinded K. A.,
Taylor N. L.,
Watkins P. J. F.,
Harrison M. E.
Publication year - 1993
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290071018
Subject(s) - chemistry , thermospray , chromatography , electrospray , mass spectrometry , dissociation (chemistry) , analytical chemistry (journal) , capillary action , electrospray mass spectrometry , ion , mass spectrum , sample preparation in mass spectrometry , collision induced dissociation , electrospray ionization , tandem mass spectrometry , selected reaction monitoring , organic chemistry , materials science , composite material
A mixture of four squalestatins was separated anil characterized by gradient packed capillary liquid chromatography combined with electrospray collision‐induced dissociation mass spectrometry (ESI/CID/MS). Negative‐ion ESI was found to afford spectra typified by strong pseudo‐molecular ions but providing little structural information. Structural information was obtained using an ion‐focusing RF‐only octapole lens to provide conditions for CID. The new method showed improvements in sensitivity when compared to existing thermospray and dynamic liquid secondary‐ion mass spectrometry methods.