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Matrix‐assisted laser desorption ionization of oligonucleotides with various matrices
Author(s) -
Tang K.,
Allman S. L.,
Chen C. H.
Publication year - 1993
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290071016
Subject(s) - chemistry , oligonucleotide , matrix (chemical analysis) , ferulic acid , matrix assisted laser desorption/ionization , ion , desorption , ionization , matrix isolation , analytical chemistry (journal) , chromatography , organic chemistry , dna , molecule , biochemistry , adsorption
Ferulic acid, 3‐hydroxypicolinic acid (3‐HPA), and 2,5‐dihydroxybenzoic acid (2,5‐DHB) were used as matrices in MALDI of various oligonucleotides. It was found that 2,5‐DHB is an excellent matrix for polydeoxyribothymi‐dylic acid. A poly‐T oligonucleotidc with a size of 130 bases was successfully detected. 3‐HPA was found to be a good matrix for MALDI of homo‐oligomeric deoxynucleotides as well as those containing four different bases. Parent ions of pd(A) 60 , d(T) 100 d(G) 40 , and d(C) 40 were observed, and a mixed‐base oligonucleotide of 150‐mer was also detected. Polymer ions of poly‐A as large as 420‐mer were measured; however, the efficiency for detecting poly‐T with 3‐HPA as a matrix was slightly worse than when 2,5‐DHB was used. Comparisons of three matrices for various oligonucleotides is also presented.