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Selected‐ion accumulation from an external electrospray ionization source with a fourier‐transform ion cyclotron resonance mass spectrometer
Author(s) -
Bruce James E.,
Anderson Gordon A.,
Hofstadler Steven A.,
van Orden Steven L.,
Sherman Michael S.,
Rockwood Alan L.,
Smith Richard D.
Publication year - 1993
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290071012
Subject(s) - fourier transform ion cyclotron resonance , chemistry , mass spectrometry , ion cyclotron resonance , quadrupole mass analyzer , ion , ion source , selected ion monitoring , analytical chemistry (journal) , electrospray ionization , hybrid mass spectrometer , ionization , atomic physics , cyclotron , tandem mass spectrometry , selected reaction monitoring , physics , chromatography , gas chromatography–mass spectrometry , organic chemistry
The coupling of an electrospray ionization (ESI) source to a Fourier‐transform ion cyclotron resonance (FTICR) mass spectrometer provides a facility for the high resolution, accurate mass analysis of large biopolymers. Typically the m/z range of the electrosprayed ions injected and trapped in the FTICR cell is between m/z 500 and 2500 (but can vary considerably with solution conditions). Recent reports on quadrupole excitation have demonstrated the ability to cool the magnetron motion of ions trapped in the FTICR mass spectrometer cell by converting this motion to cyclotron motion which, under appropriate pressure conditions, damps readily to the center of the cell. The use of a broadband waveform (swept frequency or stored waveform inverse Fourier‐transform) for the quadrupole cooling pulse was shown to provide cooling of a wide range of m/z ions, while implementation of a single frequency demonstrated a much narrower m/z response. This report demonstrates the successful combination of single‐frequency quadrupole cooling with external injection of electrosprayed ions into an FTICR mass spectrometer for m/z selected‐ion accumulation. This capability is particularly significant with electrospray ionization since it allows ions of only one charge state to be accumulated in the cell, and greatly increases the potential dynamic range of ESI‐FTICR.