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Mass spectrometric sequencing of linear peptides by product‐ion analysis in a reflectron time‐of‐flight mass spectrometer using matrix‐assisted laser desorption ionization
Author(s) -
Kaufmann R.,
Spengler B.,
Lützenkirchen F.
Publication year - 1993
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290071010
Subject(s) - reflectron , chemistry , mass spectrometry , analytical chemistry (journal) , matrix assisted laser desorption electrospray ionization , time of flight mass spectrometry , ion , time of flight , mass spectrum , ion source , ionization , field desorption , matrix assisted laser desorption/ionization , desorption , thermal ionization mass spectrometry , chromatography , adsorption , organic chemistry
In matrix‐assisted laser desorption ionization (MALDI) a large fraction of analyte ions undergo post‐source decay (PSD) during flight in the field‐free drift path. By means of a modified two‐stage reflectron, product ion time‐of‐flight spectra of medium‐sized linear peptides (up to 2800 u) were recorded, containing full sequence information. Precision, accuracy and mass resolution of fragment ions were almost as good as obtained in high‐energy collisionally activated dissociation (CAD) studies performed in four‐sector instruments. Instrumental sensitivity was better by at least one order of magnitude. In reflectron time‐of‐flight mass spectrometry (RETOF‐MS) the cleavage pattern of PSD products is different from that obtained by high‐energy and low‐energy CAD. Activation mechanisms of PSD were found to be largely determined by collisional events (ion/neutral) occurring in the acceleration field during early plume expansion. Future potentials of PSD analysis after MALDI are discussed.