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The analysis of mock DNA sequencing reactions using matrix‐assisted laser desorption/ionization mass spectrometry
Author(s) -
Fitzgerald Michael C.,
Zhu Lin,
Smith Lloyd M.
Publication year - 1993
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290071008
Subject(s) - chemistry , mass spectrometry , ionization , dna sequencing , matrix (chemical analysis) , chromatography , adduct , matrix assisted laser desorption/ionization , desorption , resolution (logic) , maldi imaging , analytical chemistry (journal) , dna , ion , organic chemistry , biochemistry , adsorption , artificial intelligence , computer science
A mock sequencing experiment is described in which matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry is used to analyze mixtures of synthetic oligodeoxynucleotides chosen to match the first 24 DNA fragments generated in typical sequencing reactions using a standard M13mp19 template and primer. The experiments permitted the evaluation of important issues relating to resolution, sensitivity, multiple charging, and adduct formation. Mass resolutions (full width at half maximum) were between 100 and 200, and it was relatively straightforward to assign bases in the sequencing simulation using subpicomolar quantities of material. These results demonstrate the potential of MALDI mass spectrometry in DNA sequence analysis.