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Taxoid side‐chain structure determination by electrospray ionization tandem mass spectrometry
Author(s) -
Bitsch Francis,
Shackleton Cedric H. L.,
Ma Wenwen,
Park Gary,
Nieder Matthew
Publication year - 1993
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290071007
Subject(s) - chemistry , fragmentation (computing) , tandem mass spectrometry , mass spectrometry , electrospray ionization , electrospray , side chain , triple quadrupole mass spectrometer , top down proteomics , tandem , protein mass spectrometry , analytical chemistry (journal) , taxoid , chromatography , selected reaction monitoring , organic chemistry , medicine , polymer , materials science , surgery , chemotherapy , computer science , paclitaxel , composite material , operating system
Abstract A method to elucidate the side‐chain structure of novel taxoids was developed through use of electrospray ionization tandem mass Spectrometry. The intact side‐chain fragments produced by source fragmentation in the atmosphere‐to‐vacuum interface were further dissociated in the collision cell of a triple quadrupole mass spectrometer. The distinctive collisionally induced dissociation mass spectra were remarkably informative and gave detailed information on the side‐chain functionalities. This technique proved valuable in the characterization of new taxoids produced by yew‐tree cell cultures.