Premium
Suppression effects on a reduction process in fast‐atom bombardment mass spectrometry
Author(s) -
Murthy Vemisetty S.,
Miller Jack M.
Publication year - 1993
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290071004
Subject(s) - chemistry , fast atom bombardment , mass spectrometry , photochemistry , reducing agent , ion , hydrogen atom , molecule , matrix (chemical analysis) , metal , radical , chemical ionization , inorganic chemistry , hydrogen , methylene , chloride , ionization , chromatography , organic chemistry , alkyl
Various methods of suppression of the reduction process occurring in fast‐atom bombardment (FAB) mass spectrometry have been studied, using the organic dyes rhodamine B and methylene blue as reference compounds. Our results indicate that transition metal chlorides have a strong influence on the suppression of the reduction process, while stannous chloride exhibits a remarkable suppression effect despite being a strong reducing agent. We suggest that the metal radicals generated in glycerol solution are responsible for these suppression effects. This phenomenou strongly supports the mechanism of hydrogen radical addition to the substrate molecules, postulated earlier as the cause of the reduction process. Co‐solvents in the matrix have also been found to suppress the reduction ions along with other matrix‐interfering ions. These studies help shed some light on the FAB ionization mechanism.