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Electrospray ionization mass spectrometry coupled with size‐exclusion chromatography
Author(s) -
Prokai Laszlo,
Simonsick William J.
Publication year - 1993
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290070914
Subject(s) - chemistry , chromatography , extractive electrospray ionization , gel permeation chromatography , electrospray ionization , mass spectrometry , electrospray , elution , size exclusion chromatography , direct electron ionization liquid chromatography–mass spectrometry interface , sample preparation in mass spectrometry , ionization , analytical chemistry (journal) , ion , chemical ionization , organic chemistry , enzyme , polymer
The coupling of electrospray ionization mass spectrometry with gel permeation chromatography (GPC) has been used for the separation of a commercial polydisperse mixture of octylphenoxypoly(ethoxy)ethanol. Stable electrospray conditions are obtained upon using, as GPC solvent, tetrahydrofuran containing 10 −5 −10 −4 M sodium salt which also provides for the formation of intact molecular ions through cationization. Selected‐ion chromatograms of sodiated species allow for the monitoring of the elution profiles of individual oligomers, and can be used for the calibration of the GPC.