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Use of an on‐line liquid chromatography trapping column for the purification of protein samples prior to electrospray mass spectrometric analysis
Author(s) -
Kay I.,
Mallet A. I.
Publication year - 1993
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290070812
Subject(s) - chemistry , chromatography , electrospray , trapping , mass spectrometry , column (typography) , column chromatography , analytical chemistry (journal) , ecology , structural engineering , connection (principal bundle) , engineering , biology
A number of reagents, commonly used in protein chemistry and purification, suppress the electrospray ionization signal response of proteins in a concentration‐dependent manner. An on‐line trapping column device has been developed which enables the purification of protein samples containing these reagents, prior to their introduction into the mass spectrometer ion source.