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Investigation of some steroids by means of thermospray and tandem mass spectrometry
Author(s) -
Curcuruto O.,
Franchi D.,
Hamdan M.,
Favretto D.,
Traldi P.
Publication year - 1993
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290070725
Subject(s) - chemistry , thermospray , mass spectrometry , tandem mass spectrometry , chromatography , tandem , analytical chemistry (journal) , ion , detection limit , estriol , selected reaction monitoring , organic chemistry , biochemistry , materials science , hormone , composite material
The detection limits of a number of steroids have been determined by means of loop‐injection thermospray (TS) mass spectrometry. The measured limits were found to be in the range 1 pg‐1O ng/μL. Combined positive‐ion TS/tandem mass spectrometry yielded clear qualitative differences between mass selected protonatcd testosterone and estriol ions, both of which have the same nominal molecular weight. These differences are necessary for the correct identification of either species which may be present within a complex mixture. The heights of the peaks due to the two competing reaction channels, [M + H] + and [M + NH 4 ] + , were monitored as a function of the repeller voltage in the range 100‐350 V, and the implications of the acquired data are discussed.