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Investigation of the metabolism of felodipine using on‐line liquid chromatography/UV diode array detection/atmospheric pressure chemical ionization mass spectrometry as a diagnostic tool
Author(s) -
Macrae P. V.,
Jones B. C.,
Todd J. F. J.
Publication year - 1993
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290070710
Subject(s) - chemistry , metabolite , atmospheric pressure chemical ionization , felodipine , chromatography , mass spectrometry , thermospray , atmospheric pressure , analytical chemistry (journal) , liquid chromatography–mass spectrometry , tandem mass spectrometry , ionization , chemical ionization , electrospray ionization , selected reaction monitoring , organic chemistry , biochemistry , ion , medicine , oceanography , blood pressure , radiology , geology
The utility of on‐line liquid chromatogaphy coupled to UV diode array detection and atmospheric pressure chemical ionization mass spectrometry for the analysis of microsomal metabolites of felodipine has been investigated. A previously unreported metabolite of felodipine, extracted from male rat microsomes, has been detected using this technique. UV diode array detection has been used to characterize this metabolite as a dihydropyridine metabolite and tandem mass spectrometry data used to assign a structure.