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Electron ionization‐tandem mass spectrometry of glycosphingolipids. Part II. The identification of a carbohydrate sequence corresponding to a novel repetitive blood group a heptaglycosylceramide
Author(s) -
Holgersson Jan,
Curtis Jonathan M.,
Morris Michael R.,
Teller Lee W.,
Derrick Peter J.,
Samuelsson Bo E.
Publication year - 1993
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290070604
Subject(s) - chemistry , mass spectrometry , tandem mass spectrometry , electron ionization , chromatography , glycosphingolipid , ionization , analytical chemistry (journal) , ion , biochemistry , organic chemistry
In a previous paper, the presence in human kidney vein tissue of a novel blood group A heptaglycosylceramide based on the type‐3 carbohydrate chain GalNAcαl‐3(Fucαl‐2)Galα1‐3GalNAcαl‐3(Fucα1‐2)Galβl‐4Glcβl‐1 Ceramide, was suggested based on thin‐layer immunostaining and electron ionization mass Spectrometry. Ions corresponding lo a structure containing two deoxyhexoses, two hexosamines and three hexoses were identified, but no information was obtained from mass spectrometry concerning the carbohydrate sequence 5 . In the present paper, we report the identification of carbohydrate sequence ions corresponding to a type‐3 chain A heptaglyco‐sylceramide by electron ionization‐tandem mass spectrometry of a permethylated‐reduced glycosphingolipid mixture isolated from human kidney vein tissue. The use of a microchannel‐plate‐array detector increased the sensitivity for collision‐induced dissociation spectra by a factor of at least ten over a conventional electron multiplier.